Advanced in vitro pulmonary models for engineered nanomaterial hazard assessment using a dry powder exposure system

Feb09Wed

Advanced in vitro pulmonary models for engineered nanomaterial hazard assessment using a dry powder exposure system

Wed, 09/02/2022 - 13:30 to 14:30
Speaker: 
David Brown
Synopsis: 

The drive to produce more relevant and robust in vitro co-culture systems to replace the use of animal exposures have resulted in the development of different types of complex systems. At the simplest level, submerged monocultures of cells do not closely match the in vivo situation and exposure to suspensions of test materials can be artificial and problematic depending on the physicochemical nature of the substance. In this investigation, we aimed to assess the suitability of the Vitrocell Dry Powder system for use in in vitro exposures of nanomaterials to co-cultures of lung cells cultured on membrane filters at the air-liquid interface (ALI). The main focus of this investigation was to optimise the dry powder system and to assess its suitability for use in in vitro long term repeat exposures.

Institute: