3D Multiplane Imaging: Full cell nanometric particle localization and tracking

Despite the remarkable advances in microscopy, in terms of resolution and imaging modalities, real time three dimensional (4D) imaging remains elusive. Crucial for understanding the complex dynamic process that occur in living cells, 4D imaging developments have been restricted to shallow depth ranges, poor spatial resolution or hugely complex, indirect imaging methodologies. Multi-plane imaging offers a simple solution by simultaneously imaging several distinct object planes. A significant advantage is that the traditional imaging modality is preserved, easing data processing and interpretation and allowing for applications in single molecule based super resolution techniques. However, multi plane imaging implies complex optical assemblies that are experimentally limiting. In this talk I will explain how one potential solution to studying the very small lay with the study of the very large: how astronomy based technologies have led to a simple, single element solution to 3D multi-plane live imaging with a direct impact for cellular imaging. When combined with analysis techniques, which also derive from astronomy, our QD based multi-plane imaging can deliver full cell, sub 10 nm accurate depth localization opening the door to full cell, 3D, super resolution imaging.