The ovine corpus luteum angiogenesis model: A tool for developing imaging technology

Robust tools for the quantitation of perfusion are not fully developed using contrast enhance ultrasound (CEUS). The ovine corpus luteum (CL) is a transient gland in the ovary that is formed to produce the hormone progesterone, but most importantly it has a well regulated angiogenic and antiangiogenic mechanism. In a number of different experiments it was shown that this property may be used to develop new imaging methodology. Using a Philips iU22 ultrasound scanner (Philips Medical Systems Corp, Seattle, WA) in contrast imaging mode it was shown that a highly controlled experiment may produce high levels of reproducibility in the transit of contrast to below than 10%. Also, compartmental kinetics models were tested. The use of prostaglanding F2alpha promotes an intense antiangiogenic process within a few hours. This is possible to monitor with CEUS prior to demise of the CL in 24 hours. Finally, the live angiogenic property of the CL during the oestrous cycle requires further investigation. In conclusion, the CL offers an attractive changing vascular bed for assessing clinically relevant perfusion imaging methodology.