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Newly emerging super-resolution imaging approaches achieve resolutions below the size of antibody-probes, which are usually used to detect unlabelled proteins within a cell. Therefore there is a strong demand for new, highly specific, smaller fluorescent probes.
Camellids and some sharks produce antibody types that are smaller than conventional antibodies. Using these special antibodies it is possible to increase the achievable spatial resolution of microscopy.
I will describe how these antibodies can be made using a viral library in vitro and how they can be used to increase the resolution of superresolution microscopy.