Integrated microfluidic platform for circulating biomarker isolation from whole blood

Circulating nucleic acids (CNAs), including fragmented DNA, RNA and miRNA, were used in several studies as reliable biomarkers in the diagnosis and monitoring of cancer progression, grafted organs rejection, NIPT and several other medical applications. Currently, the greatest obstacle to the introduction of CNAs related technologies within standard medical practice consists in sample preparation, which still requires lengthy procedures, trained staff and the use of centralised laboratories. The aim of my project is the realisation of a disposable microfluidic cartridge for the isolation and purification of CNAs from whole blood and of a portable automated platform which will allow the use of the cartridge at the point-of-care. After the developing of a fast and cost-effective method for cartridge prototyping, and the realization of several generations of cartridges to overcome fluidic issues, the project is now focusing on biological characterization and platform automation.