Label-free Separation of Tumour-delivered Microvesicles from Serological Samples Using the Deterministic Lateral Displacement Effect

Biochip-integrable sorting and separation of micron-sized particles have an increasing importance in biomedical diagnostics, biochemical analyses, food and chemical processing, and environmental assessment. By employing the unique characteristics of microscale flow phenomena, various techniques have been established for fast and accurate separation, and to sort cells or particles in a continuous manner. As in classical separation procedures, the biochip-integrable size-fractionation of particles or cells could be realized by passive or active way. Passive procedures, which do not require external force-field, utilize the interaction between particles-particle, flow-particle, and the channel structure-particle to separate different-sized particles. Meanwhile, the active separation techniques make use of external force-field in various forms.
The main target of his seminar is to give a brave introduction of the biochip-integrable separation methods for biomedical use, represent a novel biochip-integrable pathogen detection device, and a novel application of an asymmetric column structure, which called deterministic lateral displacement (DLD) device. The working principles are explained in detail, and performances of the devices are discussed with the results of the measurements.